Journal Club

AHMRN Journal Club

Working in the field of human microbiome research? 

Join us for our AHMRN Journal Club where we get to the nitty gritty of microbiome study methods and practical issues.

To keep the Journal Club on track we've put together some brief guidelines below. Please have a look at these before presenting.

Guidelines


Join our mailing list to keep up to date with upcoming presenters and to volunteer to present.


Next Journal Club

Please join us for: Detailed mapping of Bifidobacterium strain transmission from mother to infant via a dual culture-based and metagenomic approach.


Presented by: Dr Callum Walsh


Date: TBA

Time: TBA

Venue: Online

Zoom: https://adelaide.zoom.us/j/81218265426?pwd=OUlJeUNlVkhsR2ZLcW8zQWRmQm55UT09&from=addon

 

Paper: Detailed mapping of Bifidobacterium strain transmission from mother to infant via a dual culture-based and metagenomic approach


Abstract: A significant proportion of the infant gut microbiome is considered to be acquired from the mother during and after birth. Thus begins a lifelong and dynamic relationship with microbes that has an enduring impact on host health. Based on a cohort of 135 mother-infant dyads, we investigated the phenomenon of microbial strain transfer, with a particular emphasis on the use of a combined metagenomic-culture-based approach to determine the frequency of strain transfer involving members of the genus Bifidobacterium, including species/strains present at low relative abundance. From the isolation and genome sequencing of over 449 bifidobacterial strains, we validate and augment metagenomics-based evidence to reveal strain transfer in almost 50% of dyads. Factors important in strain transfer include vaginal birth, spontaneous rupture of amniotic membranes, and avoidance of intrapartum antibiotics. Importantly, we reveal that several transfer events are uniquely detected employing either cultivation or metagenomic sequencing, highlighting the requirement for a dual approach to obtain an in-depth insight into this transfer process.


Bio: Dr. Calum Walsh is a postdoctoral bioinformatician in the lab group of Prof. Tim Stinear at The Peter Doherty Institute for Infection and Immunity. His current research is centred on the interaction dynamics within the human microbiome to understand the roles of different commensal microorganisms in health and disease. Particularly in the context of multidrug resistant human pathogens such as vancomycin-resistant Enterococcus faecium and carbapenem-resistant Enterobacterales.

He has a decade of experience in the analysis of microbiome-centric datasets including 16S amplicons, shotgun metagenomics and metatranscriptomics, as well as bacterial genomes and transcriptomes.

He is also bioinformatics coordinator at Doherty Applied Microbial Genomics, a collaborative research initiative established to assist researchers, particularly those from other disciplines, use microbial genomics methods in their work.



Next Journal Club

Please join us for: Autologous Faecal Microbiota Transplantation to Improve Outcomes of Haematopoietic Stem Cell Transplantation: Results of a Single-Centre Feasibility Study 


Presented by: Ms Anna Li


Date: Mon 25 March 2024

Time: 1:00pm AEDST

Venue: Online

Zoom: https://adelaide.zoom.us/j/81218265426?pwd=OUlJeUNlVkhsR2ZLcW8zQWRmQm55UT09&from=addon

 

Paper: Biomedicines | Free Full-Text | Autologous Faecal Microbiota Transplantation to Improve Outcomes of Haematopoietic Stem Cell Transplantation: Results of a Single-Centre Feasibility Study (mdpi.com)

  
Further details to be added soon!


Previous Journal Clubs

Informal Bioinformatics Seminar

Hosted by the Monash Genomics and Bioinformatics Platform in conjunction with the Australasian Human Microbiome Research Network


Please join us for: Metagenomics and the microbiome: disentangling complex biology from complex data


Date: Wed 09 August 2023

Time: 2:30pm AEST

Venue: Bld 76, 19 Innovation Walk, level 2, room 204 (central meeting room opposite lifts),
Monash University Clayton Campus

Zoom: https://monash.zoom.us/j/86788669392?pwd=QWZDemhRbTlqYmpEYVBUSkgzbWNtZz09

 

Abstract:

Our understanding of the role and significance of the human microbiome in health and disease has been spearheaded by major developments in DNA sequencing technologies, as well as conceptual and methodological advances in the field of metagenomics. In my talk, I'll discuss the challenges of doing data-driven research on the gut microbiome, using my recent work on faecal microbiota transplantation as a case study (Science 2016, PMID:27126044; Nature Medicine 2022, PMID: 36109636).

 
Bio:
Simone is a computational biologist and NHMRC CJ Martin Research Fellow, and leads the new Microbiome Systems research group at the Biomedicine Discovery Institute at Monash University.
Trained as an engineer in Sydney at the University of New South Wales, she's happy to be back in Australia after doing her PhD in Germany (EMBL, Heidelberg) and a postdoc in Denmark (Novo Nordisk Foundation Center for Biosustainability, Copenhagen) that was supported by a competitive EMBO Fellowship. Her research has often bridged discovery and translational sciences - spanning systems biology, metagenomics and antimicrobial resistance in both clinical and biotechnology contexts.
Simone and her team will use bioinformatics, microbiomics and machine learning approaches to study microbial communities in their native ecosystems and create data-oriented methods to analyse and disentangle the biological complexity contained within them. One focus will be to look at how the microbes collectively respond to human-mediated interventions and identify ways we can leverage this information to enable improved and rational design of sustainable microbiome-based therapeutic and remediation strategies.